Vector Name:
pIEx-NcoI_cHalo_DC
Synonyms:
''
Sequencing Primer:
Forward:
pIEx Forward
Reverse:
cHaloseqR
Description:
baculovirus/insect cell transposition, with tag/fusion/marker assay, Gateway® clone, baculovirus/insect cell expression expression with 1629 and lef-2 and ORF603 and p10 promoter + 5' UTR and ie1 terminator and pUC ori and Ap and Gateway Death Cassette and ColE1 origin and F1 origin and M13 origin and LacZ alpha and CmR and attR1 and attR2 and ccdB and c-Halo and cHaloseqR and pCPDHaloseqF and TEV and hr5 and ie1 promoter and ie1 5'-UTR; ampicillin resistance in bacterial, chloramphenicol resistance in bacterial;
Comments:
CPD only
Size (bp):
9314
Parent Vector:
Name:
pIEx-cyto
Description: Insect cell expression vector with N-terminal 8xHis tag HRV3C protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
Properties:
Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, with tag/fusion/marker
Author Name:
Joshua LaBaer
Ji Qiu
Justin Saul
Ji Qiu
Justin Saul
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
-10 signal
c-Halo
null
4169
5053
5pUTR
ie1 5'-UTR
null
2229
2278
CDS
LacZ alpha
null
9246
9314
Terminator
ie1 terminator
null
5083
5389
bacterial origin
pUC ori
null
6872
7631
bacterial origin
ColE1 origin
null
7020
7702
bacterial origin
F1 origin
null
8806
9112
bacterial origin
M13 origin
null
8789
9244
death cassette
Gateway Death Cassette
null
2431
4085
negative selection marker
ccdB
null
3640
3945
primer
cHaloseqR
null
4226
4244
primer
pCPDHaloseqF
null
4933
4950
promoter
p10 promoter + 5' UTR
null
2279
2391
promoter
hr5
null
1196
1677
promoter
ie1 promoter
null
1682
2228
protease cleavage site
TEV
null
4133
4153
recombination site
1629
recombines with BacMagic DNA
5434
6852
recombination site
attR1
null
2406
2430
recombination site
attR2
null
4086
4110
recombination site
lef-2
recombines with BacMagic DNA
4
453
recombination site
ORF603
recombines with BacMagic DNA
491
1096
selectable marker
Ap
null
7800
8657
selectable marker
CmR
null
2639
3295
