Vector Name:
pET-23a
Synonyms:
pET23a
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
with tag/fusion/marker assay, multiple cloning site clone, ssDNA production expression with T7 Terminator and 6X His and T7 tag and RBS and T7 promoter and ROP and bom and ori and AmpR and AmpR promoter and f1 ori; ampicillin resistance in bacterial;
Comments:
More information available from Novagen.
Size (bp):
3666
Parent Vector:
None
Properties:
multiple cloning site, ssDNA production, with tag/fusion/marker
Author Name:
Novagen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
2208
3068
CDS
6X His
6xHis affinity tag
140
157
CDS
T7 tag
leader peptide from bacteriophage T7 gene 10
207
239
CDS
ROP
Rop protein, which maintains plasmids at low copy number
828
1019
Miscellaneous Feature
bom
basis of mobility region from pBR322
1121
1263
RBS
RBS
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
247
269
Terminator
T7 Terminator
transcription terminator for bacteriophage T7 RNA polymerase
26
73
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
301
319
promoter
AmpR promoter
null
3069
3173
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
3200
3655
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
1449
2037
