Vector Name:
pcDNA3.1zeo
Synonyms:
pcDNA3-1-zeo, pcDNA3.1-zeo
Description:
multiple cloning site clone, in vitro transcription, mammalian expression, ssDNA production expression with CMV enhancer and CMV Promoter and T7 promoter and BGH pA and f1 ori and SV40 promoter and SV40 Ori and EM7 and BseRI and SV40 poly(A) signal and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
None
Size (bp):
5015
Parent Vector:
None
Properties:
in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
235
614
poly-A signal
BGH pA
bovine growth hormone polyadenylation signal
1028
1252
promoter
CMV Promoter
human cytomegalovirus (CMV) immediate early promoter
615
818
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
863
881
promoter
SV40 promoter
SV40 enhancer and early promoter
1740
2070
promoter
EM7
synthetic bacterial promoter
2118
2165
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
1298
1726
rep_origin
SV40 Ori
SV40 origin of replication
1921
2056
