Vector Name:
pIEx-cGFP-Avi-His-DC
Synonyms:
''
Sequencing Primer:
Forward:
pIEx_seq_F
Reverse:
pIEx_seq_R
Description:
BAC mutagenesis, baculovirus/insect cell transposition, with tag/fusion/marker assay, Gateway® clone, baculovirus/insect cell expression expression with attR1 and baculovirus_recombination_region_(lef2_ORF603) and hr5 and IE1 and pIEx_seq_F and p10 promoter and lac UV5 promoter and Chloramphenicol-R and ccdB and attR2 and TEV and CyPet and AviTag and pIEx_seq_R and baculovirus_recombination_region_(ORF1629) and AmpR and AmpR promoter and f1 and 6X His and ie1 terminator; ampicillin resistance in bacterial, ampicillin resistance in insect cells;
Comments:
CPD only
Size (bp):
9198
Parent Vector:
None
Properties:
BAC mutagenesis, Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Yanyang
Justin Saul
Yanyang
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
flourescent marker
CyPet
null
4165
4860
origin
f1
null
8673
9128
peptide
AviTag
null
4864
4907
peptide
6X His
null
4915
4931
primer
pIEx_seq_F
null
2250
2269
primer
pIEx_seq_R
null
5023
5042
promoter
IE1
null
1682
2273
promoter
p10 promoter
null
2283
2392
promoter
lac UV5 promoter
null
2551
2581
promoter
AmpR promoter
null
8542
8646
protease cleavage site
TEV
null
4129
4149
recombination site
attR1
null
2402
2526
recombination site
baculovirus_recombination_region_(lef2_ORF603)
null
329
1156
recombination site
attR2
null
3982
4106
recombination site
baculovirus_recombination_region_(ORF1629)
null
5302
6659
selectable marker
Chloramphenicol-R
null
2635
3294
selectable marker
ccdB
null
3636
3941
selectable marker
AmpR
null
7681
8541
trln enhancer
hr5
null
1196
1678
trln terminator
ie1 terminator
null
4967
5273
