Vector Name:
pIEx-cHis-DC
Synonyms:
''
Sequencing Primer:
Forward:
pIEx_seq_F
Reverse:
pIEx_seq_R
Description:
BAC mutagenesis, baculovirus/insect cell transposition assay, Gateway® recombinational cloning clone, baculovirus/insect cell expression expression with baculovirus_recombination_region_(lef2_ORF603) and hr5 and IE1 and pIEx_seq_F and p10 promoter and attR1 and lac UV5 promoter and Chloramphenicol-R and ccdB and attR2 and TEV and ie1 terminator and pIEx_seq_R and baculovirus_recombination_region_(ORF1629) and Origin and AmpR and AmpR promoter and f1; ampicillin resistance in bacterial, ampicillin resistance in insect cells;
Comments:
CPD only
Size (bp):
8444
Parent Vector:
None
Properties:
BAC mutagenesis, Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, recombinational cloning
Author Name:
Joshua LaBaer
Justin Saul
Yanyang
Justin Saul
Yanyang
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
origin
Origin
null
6168
6756
origin
f1
null
7919
8374
primer
pIEx_seq_F
null
2250
2269
primer
pIEx_seq_R
null
4269
4288
promoter
IE1
null
1682
2273
promoter
p10 promoter
null
2283
2392
promoter
lac UV5 promoter
null
2551
2581
promoter
AmpR promoter
null
7788
7892
protease cleavage site
TEV
null
4129
4149
recombination site
baculovirus_recombination_region_(lef2_ORF603)
null
329
1156
recombination site
attR1
null
2402
2526
recombination site
attR2
null
3982
4106
recombination site
baculovirus_recombination_region_(ORF1629)
null
4548
5905
selectable marker
Chloramphenicol-R
null
2635
3294
selectable marker
ccdB
null
3636
3941
selectable marker
AmpR
null
6927
7787
trln enhancer
hr5
null
1196
1678
trln terminator
ie1 terminator
null
4213
4519
