Vector Name:
pIEx-nGST-DC
Synonyms:
''
Sequencing Primer:
Forward:
piex-seq-f
Reverse:
piex-seq-r
Description:
BAC mutagenesis, baculovirus/insect cell transposition assay, Gateway® clone, bacterial expression expression with baculovirus_recombination_region_(lef2_ORF603) and hr5 and IE1 and pIEx_seq_F and p10 promoter and GST and NAP138 and pGEX5 and PGEXF primer (#47) and TEV and attR1 and lac UV5 promoter and Chloramphenicol-R and ccdB and attR2 and ie1 terminator and pIEx_seq_R and baculovirus_recombination_region_(ORF1629) and Origin and AmpR and AmpR promoter and f1; ampicillin resistance in insect cells, ampicillin resistance in bacterial;
Comments:
CPD only
Size (bp):
9070
Parent Vector:
None
Properties:
BAC mutagenesis, Gateway, bacterial expression, baculovirus/insect cell transposition
Author Name:
Joshua LaBaer
Justin Saul
Yanyang
Justin Saul
Yanyang
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
origin
Origin
null
6794
7382
origin
f1
null
8545
9000
peptide
GST
null
2400
3048
primer
pIEx_seq_F
null
2250
2269
primer
NAP138
null
2502
2523
primer
pGEX5
null
3005
3027
primer
PGEXF primer (#47)
null
3024
3043
primer
pIEx_seq_R
null
4895
4914
promoter
IE1
null
1682
2273
promoter
p10 promoter
null
2283
2392
promoter
lac UV5 promoter
null
3269
3299
promoter
AmpR promoter
null
8414
8518
protease cleavage site
TEV
null
3084
3104
recombination site
baculovirus_recombination_region_(lef2_ORF603)
null
329
1156
recombination site
attR1
null
3120
3244
recombination site
attR2
null
4700
4824
recombination site
baculovirus_recombination_region_(ORF1629)
null
5174
6531
selectable marker
Chloramphenicol-R
null
3353
4012
selectable marker
ccdB
null
4354
4659
selectable marker
AmpR
null
7553
8413
trln enhancer
hr5
null
1196
1678
trln terminator
ie1 terminator
null
4839
5145
