Vector Name:
pIEx-nHis-DC
Synonyms:
''
Sequencing Primer:
Forward:
pIEx_seq_F
Reverse:
pIEx_seq_R
Description:
BAC mutagenesis, baculovirus/insect cell transposition assay, Gateway® recombinational cloning clone, baculovirus/insect cell expression expression with baculovirus_recombination_region_(lef2_ORF603) and hr5 and IE1 and pIEx_seq_F and p10 promoter and TEV and attR1 and lac UV5 promoter and Chloramphenicol-R and ccdB and attR2 and ie1 terminator and pIEx_seq_R and baculovirus_recombination_region_(ORF1629) and Origin and AmpR and AmpR promoter and f1; ampicillin resistance in bacterial, ampicillin resistance in insect cells;
Comments:
CPD only
Size (bp):
8428
Parent Vector:
None
Properties:
BAC mutagenesis, Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, recombinational cloning
Author Name:
Joshua LaBaer
Justin Saul
Yanyang
Justin Saul
Yanyang
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
origin
Origin
null
6152
6740
origin
f1
null
7903
8358
primer
pIEx_seq_F
null
2250
2269
primer
pIEx_seq_R
null
4253
4272
promoter
IE1
null
1682
2273
promoter
p10 promoter
null
2283
2392
promoter
lac UV5 promoter
null
2627
2657
promoter
AmpR promoter
null
7772
7876
protease cleavage site
TEV
null
2442
2462
recombination site
baculovirus_recombination_region_(lef2_ORF603)
null
329
1156
recombination site
attR1
null
2478
2602
recombination site
attR2
null
4058
4182
recombination site
baculovirus_recombination_region_(ORF1629)
null
4532
5889
selectable marker
Chloramphenicol-R
null
2711
3370
selectable marker
ccdB
null
3712
4017
selectable marker
AmpR
null
6911
7771
trln enhancer
hr5
null
1196
1678
trln terminator
ie1 terminator
null
4197
4503
