Vector Name:
pCellFree_c-mcherry_5800
Synonyms:
''
Description:
fluorescent marker, with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with T7 forward and T7 promoter and attR1 and ccdB and CmR and cat_promoter-var002 and attR2 and mCherry and 8xHis and pBADrev and T7 Terminator and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
5266
Parent Vector:
None
Properties:
Gateway, bacterial expression, fluorescent marker, with tag/fusion/marker
Author Name:
Shayli
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial operon
lac operator
var003
3137
3159
bacterial origin
ori
var012
3517
4105
fluorescent marker
mCherry
var007
2037
2735
negative selection marker
ccdB
var004
331
636
primer
T7 forward
null
7
26
primer
pBADrev
null
2964
2983
promoter
T7 promoter
null
7
25
promoter
cat_promoter-var002
null
1638
1740
promoter
lac promoter
var001
3163
3193
promoter
AmpR promoter
var003
5137
5241
protein binding site
CAP binding site
var002
3208
3229
recombination site
attR1
var002
160
284
recombination site
attR2
var003
1887
2011
selectable marker
CmR
var001
956
1637
selectable marker
AmpR
var016
4276
5136
tag
8xHis
var007
2744
2765
trxn termination sequence
T7 Terminator
var002
2967
2984
