Detailed Vector Information: pGL2-basic
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Luciferase reporter vector; amp resistance; restriction enzyme cloning.
Properties:
mammalian expression, multiple cloning site, ssDNA production
Type
Name
Description
Start
End
bacterial origin
ColE1
ColE1-type origin
3053
0
poly-A signal
polyA signal
polyadenylation signa sequence (for luc)
2518
2739
reporter gene
luc CDS
luciferase CDS
76
1728
selectable marker
AmpR
ampicillin resistance gene (bla)
3815
4675
ssDNA origin
f1 ori
f1 origin for ssDNA production
4807
5262