DNASU uses various primers to sequence verify gene inserts for most of the plasmids avialable in the repository. Below is a list of all vectors in DNASU along with the forward and reverse primer names and sequences for sequencing the insert. Fo a list of just primers and their sequences, visit our Primer page.
If you are interested in Sanger or Next Generation sequencing Services, please refer to our Sequencing Core website for more information.
| Vector Name | Forward Primer | Sequence | Reverse Primer | Sequence |
|---|---|---|---|---|
| p11 | T7p | AATTAATACGACTCACTATAGGG | T7t | ATGCTAGTTATTGCTCAGCGG |
| p15Tv-LIC | T7p | AATTAATACGACTCACTATAGGG | T7t | ATGCTAGTTATTGCTCAGCGG |
| p283 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| p4-Hb | Phsp | AGGTGACCACAACGACGC | ||
| p4-Ib | p4 | GGTTTAAACGGATCCAGC | ||
| p4-Sb | p4 | GGTTTAAACGGATCCAGC | ||
| p9T-c | PtetO | GGATCGTCGGCACCGTCA | ||
| p9T-H | Phsp | AGGTGACCACAACGACGC | ||
| p9T-I | PtetO | GGATCGTCGGCACCGTCA | ||
| p9T-S | PtetO | GGATCGTCGGCACCGTCA | ||
| pANT7_cGST | NAP150 | CCCATTGTATGGGATCTGATC | NAP138R | TGTTTCGCCATTTATCACCTTC |
| pANT7_nHA | NAP150 | CCCATTGTATGGGATCTGATC | NAP138R | TGTTTCGCCATTTATCACCTTC |
| pBABEneo | pBABE 5’ | CTTTATCCAGCCCTCAC | pBABE 3’ | ACCCTAACTGACACACATTCC |
| pBABEpuro | pBABE 5’ | CTTTATCCAGCCCTCAC | pBABE 3’ | ACCCTAACTGACACACATTCC |
| pBAD24sigCtermHis | PBADFOR | GCACGGCGTCACACTTTGC | RRNBTER | TCTGCGTTCTGATTTAATCTGTATCA |
| pBAD24sigNtermHis | PBADFOR | GCACGGCGTCACACTTTGC | RRNBTER | TCTGCGTTCTGATTTAATCTGTATCA |
| pBL166 | pFAB007 | GGACGCATCGTGGCCC | ||
| pBLUNT | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pBY011 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pBY011_GFP-NH3_CN | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| PCDF Bravo | T7 | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pCDF-BAD | ARAC5R | GGGATCATTTTGCGCTTCAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pCMVi-LSrCOMPTT | TTFOR | CAACAACTTCACCGTGAGC | HGHTER | ACTGGAGTGGCAACTTCCAGG |
| pCMVi-LS | LSFOR | TTCTGTCTCGTGGGGCAT | HGHTER | ACTGGAGTGGCAACTTCCAGG |
| pCMVi-UB | UBFOR | AACATCCAGAAGGAGTCA | HGHTER | ACTGGAGTGGCAACTTCCAGG |
| pcDNA3(+) | T7 | TAATACGACTCACTATAG | M13R | CAGGAAACAGCTATGACC |
| pcDNA3.1zeo | T7 | TAATACGACTCACTATAG | M13R | CAGGAAACAGCTATGACC |
| pCMV(delta1) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCMV(delta2) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCMV(delta3) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCMV(delta4) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCMV(delta5) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCMV(delta6) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCMV(WT) | pcDNAF | AGAGAACCCACTGCTTACTGGCTTATC | pcDNAR | AACTAGAAGGCACAGTCGAGGCTG |
| pCPD_nGST-SBP_DC | pCPD5698_F | AATTCATGGACGAGAAGACC | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pCPD_nHalo_cHIS_DC | HaloseqF | AAGCCTGCCTAACTGCAA | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pCPD_nHalo_DC | HaloseqF | AAGCCTGCCTAACTGCAA | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pCPD_nMBP-SBP_DC | pCPD5698_F | AATTCATGGACGAGAAGACC | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pCPD_nSBP-Halo_DC | pCPD5698_F | AATTCATGGACGAGAAGACC | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pCR4 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pCR4zeroBLUNT-TOPO | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pCW-FABAVI-LIC | pFAB007 | GGACGCATCGTGGCCC | ||
| pDB.GST | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDB.HBP | TEV | GAAAACCTGTACTTCCAG | ||
| pDB.His.MBP | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDB.His.NusA | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDB.His.TRX | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDB.TRX | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDB-HisGST | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDEST17 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pDNR-Dual | M13F | GTAAAACGACGGCCAGT | ||
| pDONR201 | pDONR201-forward | GTAACATCAGAGATTTTGAGACAC | pDONR201-reverse | TCGCGTTAACGCTAGCATGGATCTC |
| pDONR221 | U19 | GTTTTCCCAGTCACGACGT | AttP2 | CAGGAAACAGCTATGAC |
| pDONR223 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pEMB54 | pBAD forward | ATGCCATAGCATTTTTATCC | pBADrev | TAGTTATTGCTCAGCGGTGG |
| pENTR/D-TOPO | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pENTR201 | pDONR201-forward | GTAACATCAGAGATTTTGAGACAC | pDONR201-reverse | TCGCGTTAACGCTAGCATGGATCTC |
| pENTR221 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pENTR223 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pENTR223.1 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pET15Avi8HMbpTEV_NESG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15Avi8HSumoTEV_NESG | MBPSeq Forward | TCGTCAGACTGTCGATGAAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15_8HMbpTEV_NESG | MBPSeq Forward | TCGTCAGACTGTCGATGAAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15_NESG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15-6HCBPT_TF | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pET15Avi6HT_NESG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15b | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15-CBP6HT_TF | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pET15Nano6HT_NESG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET15TEV_NESG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
pET15Sumo_NESG |
T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET16b | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET16-DEST | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET21 NESG | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pET21_NESG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET23b | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET28a | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET28a-MISTIC | p28mis forward | CGAACCGCTGATCCAACTGG | p28mis reverse | GATATAGTTCCTCCTTTCAGC |
| pET28b(+) | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET28b-C-His | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET28-N-His-SUMO | T7 | TAATACGACTCACTATAG | ISFI_newrev | TTTGTTAGCAGCCGGATCTCAGTGG |
| pET28-N-His-thromb | T7 | TAATACGACTCACTATAG | ISFI_newrev | TTTGTTAGCAGCCGGATCTCAGTGG |
| pET28-N-His-thromb-C-strep | T7 | TAATACGACTCACTATAG | ISFI_newrev | TTTGTTAGCAGCCGGATCTCAGTGG |
| pET30a(+) | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET32-DEST | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET-32b-TEV | TRXF | GTTGAAAGAGTTCCTCGACG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pET46EK-LIC | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pET-DUETm | pET Upstream | ATGCGTCCGGCGTAGA | T7 terminator | TATGCTAGTTATTGCTCAG |
| pEU-E01 | pEUfor | GGTGACACTATAGAACTCA | pEUrev | GGCGCTAGCCGTAAATTCT |
| pEU-GST | GST Forward | CTTCGATAGGGTGTTTAACT | pEU Reverse | CCCGCTTCGCCGCCGCAGAAT |
| pEU-GST-FV | GST Forward | CTTCGATAGGGTGTTTAACT | pEU Reverse | CCCGCTTCGCCGCCGCAGAAT |
| pEU-His | His2 Forward | AGAAAGTTTATGAAGATCGATCTCAT | pEU Reverse | CCCGCTTCGCCGCCGCAGAAT |
| pEU-His-FV | pEU319 Forward | GTCGTTAGAACGCGGCTAC | pEU Reverse | CCCGCTTCGCCGCCGCAGAAT |
| pEU-HSBC | His2 Forward | AGAAAGTTTATGAAGATCGATCTCAT | T7 terminator | TATGCTAGTTATTGCTCAG |
| pFAB007 | pFAB007 | GGACGCATCGTGGCCC | ||
| pFASTBAC1 | pFB Forward | TATTCCGGATTATTCATACC | pFB Reverse | ACAAATGTGGTATGGCTGATT |
| pFASTBAC1-C-His-TOPO | pFB forward | TATTCCGGATTATTCATACC | pFB reverse | ACAAATGTGGTATGGCTGATT |
| pGEc1-DEST | pGEc1-DEST F | AGAGCTGGTTTAGTGAACCGTC | pGEn1-DEST R | TGACAACGGGCCACAACTCCTC |
| pGEc2-DEST | pGEc1-DEST F | AGAGCTGGTTTAGTGAACCGTC | pGEn1-DEST R | TGACAACGGGCCACAACTCCTC |
| pGEn1-DEST | pGEn3-DEST-5'F | AGCATGCACCACCACCATCACC | pGEn1-DEST R | TGACAACGGGCCACAACTCCTC |
| pGEn2-DEST | pGEn2-DEST F | GACAATCACTACCTGAGCACCA | pGEn1-DEST R | TGACAACGGGCCACAACTCCTC |
| pGEn3-DEST | pGEn3-DEST-5'F | AGCATGCACCACCACCATCACC | pGEn1-DEST R | TGACAACGGGCCACAACTCCTC |
| pGEXTEV | pGEX5 | GGGCTGGCAAGCCACGTTTGGTG | pGEX3 | CCGGGAGCTGCATGTGTCAGAGG |
| pGH | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pH3c-LIC | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pHIS8 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pHIS9 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pHM3C-LIC | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pJ2 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pJ4 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pJFT7_cHalo_DC | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_cHalo_empty | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_cHalo-3xFlag_DC | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_cHalo-3xHA_DC | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_n3xFlag-Halo_DC | HaloseqF | AAGCCTGCCTAACTGCAA | NAP138R | TGTTTCGCCATTTATCACCTT |
| pJFT7_n3xHA-Halo_DC | HaloseqF | AAGCCTGCCTAACTGCAA | NAP138R | TGTTTCGCCATTTATCACCTT |
| pJFT7_nFlag_cHalo_DC | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_nGST_CD-r2 | GSTseqF | GCAAGTATATAGCATGGCCT | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pJFT7_nGST_DC | GSTseqF | GCAAGTATATAGCATGGCCT | nap1273r | TAAACGTTCAATTGCTATTATCACG |
| pJFT7_nHA_cHalo_DC | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_nHALO_DC | HaloseqF | AAGCCTGCCTAACTGCAA | pANT1491R | TTTTTGTTTAAACTACCACTTT |
| pJFT7_nHalo_DC(r3) | HaloseqF | AAGCCTGCCTAACTGCAA | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pJFT7_nHalo_DC(r4) | HaloseqF | AAGCCTGCCTAACTGCAA | pCPD6268_R | AGCCAACTCAGCTTCCTTT |
| pJFT7_nHalo_empty | NAP150 | CCCATTGTATGGGATCTGATC | T7 terminator | TATGCTAGTTATTGCTCAG |
| pJFT7_nHIS_cHalo_DC | NAP150 | CCCATTGTATGGGATCTGATC | cHaloseqR | CAACATCGACGTAGTGCAT |
| pJFT7_nSNAP_DC | snap_484F | CTCGCCGTGAAAGAGTGG | NAP138R | TGTTTCGCCATTTATCACCTT |
| pJP1520 | JPO113 | GCGGTTTTGGCAGTACATCAATGGGCG | ||
| pJP1563 | JPO113 | GCGGTTTTGGCAGTACATCAATGGGCG | ||
| pJSP6_cHalo_DC | SP6 | catacgatttaggtgacactatag | cHaloSeqR | CAACATCGACGTAGTGCAT |
| pJSP6_nGST_DC | GSTseqF | GCAAGTATATAGCATGGCCT | pF1KseqR | CTTTCGGGCTTTGTTAGCAG |
| pJSP6_nHalo_DC | HaloseqF | AAGCCTGCCTAACTGCAA | pF1KseqR | CTTTCGGGCTTTGTTAGCAG |
| pJSP6_nHalo_empty | HaloseqF | AAGCCTGCCTAACTGCAA | pF1KseqR | CTTTCGGGCTTTGTTAGCAG |
| pJSP6_nSNAP_DC | snap_484F | CTCGCCGTGAAAGAGTGG | pF1KseqR | CTTTCGGGCTTTGTTAGCAG |
| pLDNT7_nFLAG | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pLenti4/V5-DEST | CMV forward | CGCAAATGGGCGGTAGGCGTG | V5 reverse | ACCGAGGAGAGGGTTAGGGAT |
| pLenti6.2/V5-DEST | CMV forward | CGCAAATGGGCGGTAGGCGTG | V5 reverse | ACCGAGGAGAGGGTTAGGGAT |
| pLIC-CH | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pLIC-EGFP | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pLIFE-miRNA | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pLP-DS3xFlag | M13F | GTAAAACGACGGCCAGT | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pLP-DS3xMyc | M13F | GTAAAACGACGGCCAGT | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pLP-EGFP-C1 | EGFP-C | CATGGTCCTGCTGGAGTTCGTG | EGFP-N | CGTCGCCGTCCAGCTCGACCAG |
| pLPS-3'EGFP | EGFP-C | CATGGTCCTGCTGGAGTTCGTG | EGFP-N | CGTCGCCGTCCAGCTCGACCAG |
| pMA507 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMA510 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMAL-c5e_MHAT | malE | GGTCGTCAGACTGTCGATGAAGCC | pMal | TGTCCTACTCAGGAGAGCGTTCAC |
| pMAPLe3 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| MBP-MPR-TM | MBP Seq Forward | TCGTCAGACTGTCGATGAAG | PQEREV | GTTCTGAGGTCATTACTGG |
| pMCentr1 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pMCentr2 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pMCentr3 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
| pMCSG10 | T7short | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG11 | DuetUp1 | GGATCTCGACGCTCTCCCT | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG12 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG13 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG14 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG17 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG18 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG19 | ANForward | CGTTTCCAGTCTCACCACC | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG19B | ANForward | CGTTTCCAGTCTCACCACC | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG19C | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG20 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG21 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG22 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG23 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG24 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG26 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG28 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG29 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG32 | T7short | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG48 | pMCSG48F | GAAGGGTTGACCGACGAAAAAGC | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG49 | T7 short | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG51 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG53 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG54 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG55 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG56 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG57 | T7short | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG58 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG59 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG60 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG62 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG63 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG68 | T7short | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG69 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG7 | T7short | TAATACGACTCACTATAGGG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMCSG70 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG71 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG73 | MCSG73F | TATGGCTGCCCGTAATAACAACAAC | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG75 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG76 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG77 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG8 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMCSG9 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pMH1 | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GATTTAATCTGTATCAGG |
| pMH1TEV | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pMH2T7 | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pMH2T7TEV | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pMH4 | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pMH4TEV | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pMHTDelta238 | pMHTDelta238-F | ACGCCCAGAAAGGTGAAATCATGC | pMHTDelta238-R | TGAGTCGCTTCCTTAACTGGCTGA |
| pMIS2.1mv | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pMKQ | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pMYC4-H | Phsp | AGGTGACCACAACGACGC | ||
| pMYC4-Hp | Phsp | AGGTGACCACAACGACGC | ||
| pMYC4-I | Ps-Pimyc | CTGTTTAAACGGATCGTCG | ||
| pMYC4-S | Ps-Pimyc | CTGTTTAAACGGATCGTCG | ||
| pMYC5-H | Phsp | AGGTGACCACAACGACGC | ||
| pNIC28-Bsa4 | pLICf | TGTGAGCGGATAACAATTCC | pLIC rev | AGCAGCCAACTCAGCTTCC |
| pNYCOMPS1 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pNYCOMPS-LIC-FH10T+ (N term) | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pNYCOMPS-TH-ccdB (C-term.) | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pNYSGXRC | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pPic3.5K | pPIC3-5F | GACTGGTTCCAATTGACAAGC | pPIC3-5R | GCAAATGGCATTCTGACATCC |
| pPic3.5K | pPIC3-5F | GACTGGTTCCAATTGACAAGC | pPIC3-5R | GCAAATGGCATTCTGACATCC |
| pPICZa | AOX1 | GACTGGTTCCAATTGACAAGC | pAOX1 Reverse | GCAAATGGCATTCTGACATCC |
| pRETRO-SUPER | pSUPER-for | GGAAGCCTTGGCTTTTG | ||
| pRSET-C6xHis | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pRSET-C8xHis | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
pRSET-natGFP |
T7 | TAATACGACTCACTATAG | GFPR | TCTCCACTGACAGAAAATTTGTGC |
| pRSET-natGFPHis | T7 | TAATACGACTCACTATAG | GFPR | TCTCCACTGACAGAAAATTTGTGC |
| pRSET-natTEVGFPHis | T7 | TAATACGACTCACTATAG | GFPR | TCTCCACTGACAGAAAATTTGTGC |
| pRSET-YtfM-8xHis-N-term | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pRSET-YtfM-AN | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pRSF/TEV/LIC | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pSB2 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pSB3 | T7 | TAATACGACTCACTATAG | T7 terminator | GCTAGTTATTGCTCAGCGG |
| pSFV3 | pFAB007 | GGACGCATCGTGGCCC | ||
| pSFV4 | pFAB007 | GGACGCATCGTGGCCC | ||
| pSGP17 | pSGP-Forward | CAACATTTTCAGTTTGTATTACTTC | pSGP-Reverse | CAATCAACTATCAACTATTAACTA |
| pSGP18 | pSGP18for | CACCTGTGCCGAAACGCAAATG | ||
| pSGP36 | pSGP36 forward | CAACATTTTCAGTTTGTATTACTTC | pSGP36 reverse | CAAGGCGATTAAGTTGGGTAACGC |
| pSGP40 | pSGP-Forward | CAACATTTTCAGTTTGTATTACTTC | pSGP-Reverse | CAATCAACTATCAACTATTAACTA |
| pSGP46 | pSGP-Forward | CAACATTTTCAGTTTGTATTACTTC | pSGP-Reverse | CAATCAACTATCAACTATTAACTA |
| pSGP47 | pSGP-Forward | CAACATTTTCAGTTTGTATTACTTC | pSGP-Reverse | CAATCAACTATCAACTATTAACTA |
| pSGX2 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX3 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX4 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX4 BS | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX5 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX6 | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX6 SP | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pSGX-TOPO | pFB forward | TATTCCGGATTATTCATACC | pFB reverse | ACAAATGTGGTATGGCTGATT |
| pSpeedE | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pSpeedE5T | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pSpeedEDT | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pSpeedEGST | GST Forward | CTTCGATAGGGTGTTTAACT | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pSpeedENATS | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pSpeedET | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pSpeedETS | pBAD forward | ATGCCATAGCATTTTTATCC | alt_bad_rev | GGGTCAGGTGGGACCAC |
| pTYB12-Intein | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| pVP13 | MBP Forward | CTACAGGCGAAAGACCATACG | pQE Reverse | CAAGACTCCAGTAATGACC |
| pVP16 | MBP Forward | CTACAGGCGAAAGACCATACG | pQE Reverse | CAAGACTCCAGTAATGACC |
| pVP33A | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP33K | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP56A | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP56K | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP65K | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP68K | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP80K | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| pVP81K | MBP Forward | CTACAGGCGAAAGACCATACG | T7 Terminator | TATGCTAGTTATTGCTCAG |
| RH2.2 | pFAB007 | GGACGCATCGTGGCCC | ||
| Topo Wild | T7 | TAATACGACTCACTATAG | T7 terminator | TATGCTAGTTATTGCTCAG |
| ZM552 | M13F | GTAAAACGACGGCCAGT | M13R | CAGGAAACAGCTATGACC |
