Gene:
Gene Symbol:
RBL2
Gene Name:
retinoblastoma-like 2 (p130)
Original Clone ID:
HsCD00298945
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00616865
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3420nts
Open reading frame: 109 to 3528
AGCGGGCAGGCGCGGTGCGGGCGGCGGACGGGCGGGCGCTTCGCCGTTTGAATGGCTGCG
GGCCCGGGCCCTCACCTCACCTGAGGTCCGGCCGCCCAGGGGTGCGCTATGCCGTCGGGA
GGTGACCAGTCGCCACCGCCCCCGCCTCCCCCTCCGGCGGCGGCAGCCTCGGATGAGGAG
GAGGAGGACGACGGCGAGGCGGAAGACGCCGCGCCGCCTGCCGAGTCGCCCACCCCTCAG
ATCCAGCAGCGGTTCGACGAGCTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGGGCC
GAGGCCTGGGACAGCTACCGCAGCATGAGCGAAAGCTACACGCTGGAGGGAAATGATCTT
CATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAAAATCTGTTCCAACTGTAAGCAAA
GGGACAGTGGAAGGAAACTATGTATCTTTAACTAGAATCCTGAAATGTTCAGAGCAGAGC
TTAATCGAATTTTTTAATAAGATGAAGAAGTGGGAAGACATGGCAAATCTACCCCCACAT
TTCAGAGAACGTACTGAGAGATTAGAAAGAAACTTCACTGTTTCTGCTGTAATTTTTAAG
AAATATGAACCCATTTTTCAGGACATCTTTAAATACCCTCAAGAGGAGCAACCTCGTCAG
CAGCGAGGAAGGAAACAGCGGCGACAGCCCTGTACTGTGTCTGAAATTTTCCATTTTTGT
TGGGTGCTTTTTATATATGCAAAAGGTAATTTCCCCATGATTAGTGATGATTTGGTCAAT
TCTTATCACCTGCTGCTGTGTGCTTTGGACTTAGTTTATGGAAATGCACTTCAGTGTTCT
AATCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCTTATCTGAAGATTTTCATGCTAAA
GATTCTAAACCTTCCTCTGACCCCCCTTGTATCATTGAGAAACTGTGTTCCTTACATGAT
GGCCTAGTTTTGGAAGCAAAGGGGATAAAGGAACATTTCTGGAAACCCTATATTAGGAAA
CTTTATGAAAAAAAGCTCCTTAAGGGAAAAGAAGAAAATCTCACTGGGTTTCTAGAACCT
GGGAACTTTGGAGAGAGTTTTAAAGCCATCAATAAGGCCTATGAGGAGTATGTTTTATCT
GTTGGGAATTTAGATGAGCGGATATTTCTTGGAGAGGATGCTGAGGAGGAAATTGGGACT
CTCTCAAGGTGTCTGAACGCTGGTTCAGGAACAGAGACTGCTGAAAGGGTGCAGATGAAA
AACATCTTACAGCAGCATTTTGACAAGTCCAAAGCACTTAGAATCTCCACACCACTAACT
GGTGTTAGGTACATTAAGGAGAATAGCCCTTGTGTGACTCCAGTTTCTACAGCTACGCAT
AGCTTGAGTCGTCTTCACACCATGCTGACAGGCCTCAGGAATGCACCAAGTGAGAAACTG
GAACAGATTCTCAGGACATGTTCCAGAGATCCAACCCAGGCTATTGCTAACAGACTGAAA
GAAATGTTTGAAATATATTCTCAGCATTTCCAGCCAGACGAGGATTTCAGTAATTGTGCT
AAAGAAATTGCCAGCAAACATTTTCGTTTTGCGGAGATGCTTTACTATAAAGTATTAGAA
TCTGTTATTGAGCAGGAACAAAAAAGACTAGGAGACATGGATTTATCTGGTATTCTGGAA
CAAGATGCGTTCCACAGATCTCTCTTGGCCTGCTGCCTTGAGGTCGTCACTTTTTCTTAT
AAGCCTCCTGGGAATTTTCCATTTATTACTGAAATATTTGATGTGCCTCTTTATCATTTT
TATAAGGTGATAGAAGTATTCATTAGAGCAGAAGATGGCCTTTGTAGAGAGGTGGTAAAA
CACCTTAATCAGATTGAAGAACAGATCTTAGATCATTTGGCATGGAAACCAGAGTCTCCA
CTCTGGGAAAAAATTAGAGACAATGAAAACAGAGTTCCTACATGTGAAGAGGTCATGCCA
CCTCAGAACCTGGAAAGGGCAGATGAAATTTGCATTGCTGGCTCCCCTTTGACTCCCAGA
AGGGTGACTGAAGTTCGTGCTGATACTGGAGGACTTGGAAGGAGCATAACATCTCCAACC
ACATTATACGATAGGTACAGCTCCCCACCAGCCAGCACTACCAGAAGGCGGCTATTTGTT
GAGAATGATAGCCCCTCTGATGGAGGGACGCCTGGGCGCATGCCCCCACAGCCCCTAGTC
AATGCTGTCCCTGTGCAGAATGTATCTGGGGAGACTGTTTCTGTCACACCAGTTCCTGGA
CAGACTTTGGTCACCATGGCAACCGCCACTGTCACAGCCAACAATGGGCAAACGGTAACC
ATTCCTGTGCAAGGTATTGCCAATGAAAATGGAGGGATAACATTCTTCCCTGTCCAAGTC
AATGTTGGGGGGCAGGCACAAGCTGTGACAGGCTCCATCCAGCCCCTCAGTGCTCAGGCC
CTGGCTGGAAGTCTGAGCTCTCAACAGGTGACAGGAACAACTTTGCAAGTCCCTGGTCAA
GTGGCCATTCAACAGATTTCCCCAGGTGGCCAACAGCAGAAGCAAGGCCAGTCTGTAACC
AGCAGTAGTAATAGACCCAGGAAGACCAGCTCTTTATCGCTTTTCTTTAGAAAGGTATAC
CATTTAGCAGCTGTCCGCCTTCGGGATCTCTGTGCCAAACTAGATATTTCAGATGAATTG
AGGAAAAAAATCTGGACCTGCTTTGAATTCTCCATAATTCAGTGTCCTGAACTTATGATG
GACAGACATCTGGACCAGTTATTAATGTGTGCCATTTATGTGATGGCAAAGGTCACAAAA
GAAGATAAGTCCTTCCAGAACATTATGCGTTGTTATAGGACTCAGCCGCAGGCCCGGAGC
CAGGTGTATAGAAGTGTTTTGATAAAAGGAAAAAGAAAAAGAAGAAATTCTGGCAGCAGT
GATAGCAGAAGCCATCAGAATTCTCCAACAGAACTAAACAAAGATAGAACCAGTAGAGAC
TCCAGTCCAGTTATGAGGTCAAGCAGCACCTTGCCAGTTCCACAGCCCAGCAGTGCTCCT
CCCACACCTACTCGCCTCACAGGTGCCAACAGTGACATGGAAGAAGAGGAGAGGGGAGAC
CTCATTCAGTTCTACAACAACATCTACATCAAACAGATTAAGACATTTGCCATGAAGTAC
TCACAGGCAAATATGGATGCTCCTCCACTCTCTCCCTATCCATTTGTAAGAACAGGCTCC
CCTCGCCGAATACAGTTGTCTCAAAATCATCCTGTCTACATTTCCCCACATAAAAATGAA
ACAATGCTTTCTCCTCGAGAAAAGATTTTCTATTACTTCAGCAACAGTCCTTCAAAGAGA
CTGAGAGAAATTAATAGTATGATACACACAGGAGAAACTCCTACTAAAAAGAGAGGAATT
CTTTTGGAAGATGGAAGTGAATCACCTGCAAAAAGAATTTGCCCAGAAAATCATTCTGCC
TTATTACGCCGTCTCCAAGATGTAGCTAATGACCGTGGTTCCCACTGAGGTTAGTCTCTT
GTATTAAACTCTTCACAAAATCTGTTTAGCAGCAGCCTTTAATGCATCTAGATTATGGAG
CTTTTTTCCTTAATCCAGCTGATGAGTTACAGCCTGTTAGTAACATGAGGGGACATTTTG
GTGAGAAATGGGACTTAACTCCTTCCAGTGTCCTTAGAACATTTTAATTCATCCCAACTG
TCTTTTTTTCCCTACCATTCAGTGATTACTGTCAAGGCTGCTTAGAATCCAAACTTGGAT
TTTTGACTCTGGCAAAGCTTTTAGAAATACTGCAAGAAAATGATGTGTACCCAAACGTGA
GCATAGGAGGCTTCTGTTGACGTACTCCAACAGAAGAACTGTTTTCAAGTTCAATCCTAC
CTGTTTTGTGGTCAGCTGTAGTCCTCATAAAAAGCAAAACAAAAATTAGGTATTTTGTCC
TAAAACACCTGGTAGGAGTGTGTGATTTTTTGCATTCCTGACAAAGGAGAGCACACCCAG
GTTTGGAGGTCCTAGGTCATTAGCCCTCGTCTCCCGTTCCCTTTGTGCACATCTTCCCTC
TCCCCATTCGGTGTGGTGCAGTGTGAAAAGTCCTTGATTGTTCGGGTGTGCAATGTCTGA
GTGAACCTGTATAAGTGGAGGCACTTTAGGGCTGTAAAATGCATGATTTTGTAACCCAGA
TTTTGCTGTATATTTGTGATAGCACTTTCTACAATGTGAACTTTATTAAATACAAAACTT
CCAGGCTAAACATCCAATATTTTCTTTAATGCTTTTATATTTTTTTAAAATGTTAAAACC
CCTATAGCCACCTTTTGGGAATGTTTTAAATTCTCCAGTTTTTTGTTATATAGGGATCAA
CCAGCTAAGAAAAGATTTTAATCAAGTTGAATTGAGGGGATTAATATGAAAACTTATGAC
CTCTTCCTTTAGGAGGGAGTTATCTAAAAGAAATGTCTATTAAGGTGATATATTTAAAAA
TATTTTTGGGTGTTCCTGGCAGTTTAAAAAAATTGGTTGGAGAATTTAGGTTTTTATTAG
TACCATAGTACCATTTATACAAATTAGAAAATGTTATTTAACAGCTGAATTATCTATACA
TATCTTTATTAATCACTATTGTTCCAGCAGTTTTCAAGTCAAATTAATAATCTTATTAGG
GAGAAAATTCAATTGTAAATTGAATCAGTATAAACAAAGTTACTAGGTAACTTCATATTG
CTGAGAGAAATATGGAACTTACATTGTTCAATTAGAATAGTGTTCTGCAAAAATATTTAT
AAAACTTCTCAAGATACTGCTACTGTAATTTTATATGAAGATAAGTGTATTTTTCAATAA
AGCATTTATAAATTAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDE AARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKC SEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEE QPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNA LQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKP YIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEE EIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVS TATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDF SNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVV TFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWK PESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSI TSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVT PVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPL SAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFF RKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMA KVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDR TSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTF AMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNS PSKRLREINSMIHTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
